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KMID : 0362720210590110640
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Journal of the Korean Dental Association
2021 Volume.59 No. 11 p.640 ~ p.648
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The effect of JD005 compound on odontogenic differentiation of human dental pulp stem cells
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Kim Da-Sol
Lee Nam-Geol
Jang Hye-Ock
Ahn Jin-Hee
Kim Hyung-Ryong
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Abstract
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Preservation of natural teeth and their structure is critical for chewing, swallowing, and better speech. Dentin and dental tissues are crucial in maintaining natural teeth and their structure, but treatment of dental tissue and dentin fracture is challenging for dentistry. Hence, scientific understanding needs to be translated to discover novel dental treatments. Dental pulp stem cells (DPSCs) are mesenchymal stem cells (MSCs) isolated from the third molar pulp. These cells have similar characteristics to MSCs with a unique property where DPSCs can differentiate into dentin forming odontoblast-like cells. Here, we made an effort to evaluate the potential substrate that induces odontoblastic differentiation of DPSCs. In this study, JD005, a modified A2A antagonist from ZM-241385, was determined to enhance OD. Specifically, JD005 treatment significantly enhanced the OD markers like alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), OPN, dentin sialophosphoprotein (DSPP), and dentin matrix protein 1 (DMP1). Additionally, the DPSCs treated with JD005 showed enhanced SMAD phosphorylation associated with differentiation. These observations distinctively suggest that JD005 potentially contributes to dentin regeneration via odontoblast differentiation.
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KEYWORD
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DPSCs, JD005, dentine regeneration, odontoblasts, RUNX2, DSPP, SMAD phosphorylation
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